2024 UPGG Computational Bootcamp

Duke University

Aug 21-23, 2024

9:00am - 3:30pm

Instructors: Odmaa Bayaraa, Natalie Dzikowski, Angela Jones, Gabriel Kennedy, Yanting "Raven" Luo, Krista Pipho, RP Pornmongkolsuk, Kayla Wilhoit

Helpers: (same as instructors)

General Information

Welcome to the UPGG Computational Bootcamp!!

As genetic techonology advances and the demand for analyzing larger data grows, it is pivotal to develop some background in computational skills. You need basic command line knowledge to operate powerful bioinformatic software and tools, as well as some coding to make both impactful and aesthetically appealing visualization of your research. In this bootcamp, we will touch on basic command lines in Unix and how to manipulate and visualize data in R. Since the best way to learn programing is to try it yourself, we focus on live-coding and have prepared a group project on the last day in which you can explore and utilize your skills freely! Lastly, this bootcamp is mostly student-run and is instructed by peer instructors -- PhD students in UPGG. All of us were, at one point, sitting where you are!

This course website was built in courtesy of The Carpentries project.

Who: Incoming class of 2024 UPGG PhD students! You don't need to have any previous knowledge of the tools that will be presented at the bootcamp.

Where: Wed, Aug 21: MSRB III Room 1125
Thurs-Fri, Aug 22-23: GSRB1 Room 4002 (Front sign says Snyderman Genome Science Research Building). Get directions to GSRB1 and MSRB III.

When: Aug 21-23, 2024. Add to your calendar.

Requirements: Participants must bring a laptop with a Mac, Linux, or Windows operating system (not a tablet, Chromebook, etc.) that they have administrative privileges on. They should have a few specific software packages installed (listed below).

Accessibility: We are committed to making this bootcamp accessible to everybody.

Please get in touch (using contact details below) if we can help making learning easier for you, and we will attempt to provide them.

Contact: Please email amanda.shipp@duke.edu or rp280@duke.edu for more information.


Collaborative Notes

We will use this collaborative document for chatting, taking notes, and sharing URLs and bits of code.


Surveys

Please be sure to complete the survey after each lesson and the overall bootcamp.

Bootcamp Survey


Schedule

Day 1

(MSRBIII 1125)

Before Welcoming words from Allison Ashley-Koch
09:00 Introduction to the Unix Shell
11:45 Lunch break
12:45 Introduction to R and R Markdown
15:30 END

Day 2

(GSRB1 4002 - Note location change!!)

09:00 Data Manipulation and Organization in R
11:45 Lunch break
12:45 Introduce student committees by co-chairs
12:50 Data Visualization in R
15:30 END

Day 3

(GSRB1 4002)

09:00 Group Project
11:45 Lunch break
12:45 Group Project Presentation
13:45 Resources
14:30 END

Syllabus

Introduction to UNIX+Data/Project Organization

  • Intro to Shell
  • Navigating Files and Directories
  • Writing Scripts and Working with Data
  • Project Organization
  • Exercise Instructions
  • Slides: PDF Link

Introduction to R and Rmd

Data exploration and cleanup

Data visualization

Group Project

Resources


Setup

To participate in a Software Carpentry workshop, you will need access to software as described below. In addition, you will need an up-to-date web browser.

We maintain a list of common issues that occur during installation as a reference for instructors that may be useful on the Configuration Problems and Solutions wiki page.

The Bash Shell

Bash is a commonly-used shell that gives you the power to do tasks more quickly.

  1. Download the Git for Windows installer.
  2. Run the installer and follow the steps below:
    1. Click on "Next" four times (two times if you've previously installed Git). You don't need to change anything in the Information, location, components, and start menu screens.
    2. From the dropdown menu, "Choosing the default editor used by Git", select "Use the Nano editor by default" (NOTE: you will need to scroll up to find it) and click on "Next".
    3. On the page that says "Adjusting the name of the initial branch in new repositories", ensure that "Let Git decide" is selected. This will ensure the highest level of compatibility for our lessons.
    4. Ensure that "Git from the command line and also from 3rd-party software" is selected and click on "Next". (If you don't do this Git Bash will not work properly, requiring you to remove the Git Bash installation, re-run the installer and to select the "Git from the command line and also from 3rd-party software" option.)
    5. Select "Use bundled OpenSSH".
    6. Ensure that "Use the native Windows Secure Channel Library" is selected and click on "Next".
    7. Ensure that "Checkout Windows-style, commit Unix-style line endings" is selected and click on "Next".
    8. Ensure that "Use Windows' default console window" is selected and click on "Next".
    9. Ensure that "Default (fast-forward or merge) is selected and click "Next"
    10. Ensure that "Git Credential Manager" is selected and click on "Next".
    11. Ensure that "Enable file system caching" is selected and click on "Next".
    12. Click on "Install".
    13. Click on "Finish" or "Next".
  3. If your "HOME" environment variable is not set (or you don't know what this is):
    1. Open command prompt (Open Start Menu then type cmd and press Enter)
    2. Type the following line into the command prompt window exactly as shown:

      setx HOME "%USERPROFILE%"

    3. Press Enter, you should see SUCCESS: Specified value was saved.
    4. Quit command prompt by typing exit then pressing Enter

This will provide you with both Git and Bash in the Git Bash program.

Video Tutorial

The default shell in Mac OS X Ventura and newer versions is Zsh, but Bash is available in all versions, so no need to install anything. You access Bash from the Terminal (found in /Applications/Utilities). See the Git installation video tutorial for an example on how to open the Terminal. You may want to keep Terminal in your dock for this workshop.

To see if your default shell is Bash type echo $SHELL in Terminal and press the Return key. If the message printed does not end with '/bash' then your default is something else, you can change your current shell to Bash by typing bash and then pressing Return. To check your current shell type echo $0 and press Return.

To change your default shell to Bash type chsh -s /bin/bash and press the Return key, then reboot for the change to take effect. To change your default back to Zsh, type chsh -s /bin/zsh, press the Return key and reboot. To check available shells, type cat /etc/shells.

Video Tutorial

The default shell is usually Bash and there is usually no need to install anything.

To see if your default shell is Bash type echo $SHELL in Terminal and press the Return key. If the message printed does not end with '/bash' then your default is something else, you can change your current shell to Bash by typing bash and then pressing Return. To check your current shell type echo $0 and press Return.

To change your default shell to Bash type chsh -s /bin/bash and press the Return key, then reboot for the change to take effect. To change your default back to Zsh, type chsh -s /bin/zsh, press the Return key and reboot. To check available shells, type cat /etc/shells.

Git

Git is a version control system that lets you track who made changes to what when and has options for easily updating a shared or public version of your code on github.com. You will need a supported web browser.

You will need an account at github.com for parts of the Git lesson. Basic GitHub accounts are free. We encourage you to create a GitHub account if you don't have one already. Please consider what personal information you'd like to reveal. For example, you may want to review these instructions for keeping your email address private provided at GitHub.

For macOS, install Git for Mac by downloading and running the most recent "mavericks" installer from this list. Because this installer is not signed by the developer, you may have to right click (control click) on the .pkg file, click Open, and click Open on the pop up window. After installing Git, there will not be anything in your /Applications folder, as Git is a command line program. For older versions of OS X (10.5-10.8) use the most recent available installer labelled "snow-leopard" available here.

Video Tutorial

If Git is not already available on your machine you can try to install it via your distro's package manager. For Debian/Ubuntu run sudo apt-get install git and for Fedora run sudo dnf install git.

Text Editor

When you're writing code, it's nice to have a text editor that is optimized for writing code, with features like automatic color-coding of key words. The default text editor on macOS and Linux is usually set to Vim, which is not famous for being intuitive. If you accidentally find yourself stuck in it, hit the Esc key, followed by :+Q+! (colon, lower-case 'q', exclamation mark), then hitting Return to return to the shell.

nano is a basic editor and the default that instructors use in the workshop. It is installed along with Git.

nano is a basic editor and the default that instructors use in the workshop. See the Git installation video tutorial for an example on how to open nano. It should be pre-installed.

Video Tutorial

nano is a basic editor and the default that instructors use in the workshop. It should be pre-installed.

R

R is a programming language that is especially powerful for data exploration, visualization, and statistical analysis. To interact with R, we use RStudio.

Install R by downloading and running this .exe file from CRAN. Also, please install the RStudio IDE. Note that if you have separate user and admin accounts, you should run the installers as administrator (right-click on .exe file and select "Run as administrator" instead of double-clicking). Otherwise problems may occur later, for example when installing R packages.

Video Tutorial

Instructions for R installation on various Linux platforms (debian, fedora, redhat, and ubuntu) can be found at <https://cran.r-project.org/bin/linux/>. These will instruct you to use your package manager (e.g. for Fedora run sudo dnf install R and for Debian/Ubuntu, add a ppa repository and then run sudo apt-get install r-base). Also, please install the RStudio IDE.